Fig. 2From: Continuous low-intensity ultrasound attenuates IL-6 and TNFα-induced catabolic effects and repairs chondral fissures in bovine osteochondral explantsCell viability of explants. The viability of cells at the cartilage interfacial region at the vicinity of the chondral incision in osteochondral explants was viewed by live-dead staining on day 3 and day 21 of culture. Explants (n = 3 per group) were incubated in 6 μM calceinAM and 4 μM ethidium homodimer-1 solution for 1 h at 37 °C under aseptic conditions. Confocal micrographs viewed from the top or superficial zone of cartilage depict live (green) cells and dead (red) cells at 10× magnification. White arrowheads indicate the leading edge of the chondral incision. Scale bar represents 200 μmBack to article page