Fig. 4

Pirfenidone significantly reduced TGF-β1-induced phosphorylation of MLC in DD-derived fibroblasts. a CT- and DD-cord derived fibroblasts derived from three different patient samples (N = 3/group) were maintained in α-MEM medium containing 0.1% dialyzed FBS for 24 h. After 24 h, cells were either treated or untreated with PFD (800 μg/ml) and in the presence or absence of TGF-β1 (10 ng/ml) for additional 24 h. Cell lysates were subjected to Western blot analyses to determine the expression of phosphorylated MLC. b Densitometry results are reported as the ratio of phosphorylated MLC protein level to GAPDH expression. Values are means ± standard error mean (SEM) of three independent studies from each of CT- and DD- derived fibroblasts. Shown here is a representative image of Western blots from three different cultures of CT- and DD-cord derived fibroblasts, each showing similar results. *p < 0.02,. Ntx;No treatment, PFD- Pirfenidone, TGF-β1, T + P; TGF- β1 + Pirfenidone