Rare variant of HSPG2 is not involved in the development of adolescent idiopathic scoliosis: evidence from a large-scale replication study

Table 1 Primers for the genotyping assay and expression analysis

	Primers
p.Asn786Ser
TC	TTTTTTTTTTTTTTTTGTTGCACTGTGGCCCCTCCtTGC
TT	TTTTTTTTTTTTTTTTTTTGTTGCACTGTGGCCCCTCCtTGT
TR	TGTGCTGGCAATTCTAGAAGAAGGAtttttt
GAPDH	TTTTTTTTTAGCTGCAGATCCTGCACCAAGAGC
Forward	5′- GAGTCAACGGATTTGGTCGT − 3’
Reverse	5′- TTGATTTTGGAGGGATCTCG − 3’
HSPG2
Forward	5′ - TACACACGCCACCTGATCTC -3’
Reverse	5′ - GCTGCCAGTAGAAGGACTCA −3’

ISSN: 1471-2474