Table 1 Gene primer information
Gene Name | Sequence (5′ - > 3′) | Annealing Temp | Amplicon length | NCBI Number |
|---|---|---|---|---|
Cartilage Matrix | ||||
Collagen, Type I, Alpha 1 (Col1a1) | F: CAACCGCTTCACCTACAGC | |||
R: TTTTGTATTCGATCACTGTCTTGCC | 60 | 101 | AK236626 | |
Collagen, Type II, Alpha 1 (Col2a1) | F: GAGAGGTCTTCCTGGCAAAG | |||
R: AAGTCCCTGGAAGCCAGAT | 60 | 118 | AF201724.1 | |
Aggrecan (Acan) | F: TGCAGGTGACCATGGCC | |||
R: CGGTAATGGAACACAACCCCT | 60 | 79 | AF201722b | |
SRY (sex determining gene region Y) box-9 (Sox9) | F: CAGGGCTCTGTGCTCTACTCC | |||
R: GGGTTACGGTCTTTCTTCGGT | 60 | 230 | NM_213843.1 | |
Osteopontin (Opn) | F: CCGCAGCCAGGAGCAGTC | |||
R: GTTGATCTCAGAAGACGCACTCTC | 55 | 214 | NM_214023.1 | |
Cartilage oligometric matrix protein (Comp) | F: GGCTGGAAGGACAAGACATC | |||
R: CCTCATAGAACCGCACTCTG | 55 | 82 | XM_003123529.1 | |
Degradative Enzymes & Inhibitors | ||||
Matrix metalloprotease-1 (Mmp1) | F: TGATGGACCTGGAGGAAACC | |||
R: GAGCAGCCACACGATACAAG | 59 | 131 | NM_001166229 | |
Matrix metalloprotease-3 (Mmp3) | F: GATGTTGGTTACTTCAGCAC | |||
R: ATCATTATGTCAGCCTCTCC | 50 | 197 | NM_001166308.1 | |
Matrix metalloprotease-13 (Mmp13) | F: CCAAAGGCTACAACTTGTTTCTTG | |||
R: TGGGTCCTTGGAGTGGTCAA | 60 | 77 | AF069643 | |
TIMP Metallopeptidase Inhibitor-1 (Timp1) | F: CCTCGTACCAGCGTTATG | |||
R: CGTTCCACAGTTGTCCAG | 59 | 177 | NM_213857.1 | |
TIMP Metallopeptidase Inhibitor-2 (Timp2) | F: ATATACGAGAACACCAGACC | |||
R: GGAATGATTACAACGGATGC | 59 | 152 | AK237154.1 | |
ADAM Metallopeptidase with Thrombospondin Type 1 Motif 5 (Adamts5) | F: CGCTGCCACCACACTCAA | |||
R: CGTAGTGCTCCTCATGGTCATCT | 60 | 80 | NM_007038.3 | |
Inflammatory Response | ||||
Indian Hedgehog (Ihh) | F: CAGCGGGCGCTATGAAGGCA | |||
R: GGTCCTTGCAGCGCTGGGTC | 60 | 140 | XM_001925486.1 | |
Transforming growth factor β (Tgfb) | F: GGAGTGGCTGTCCTTTGATGT | |||
R: AGTGTGTTATCTTTGCTGTCA | 60 | 117 | NM_214015.1 | |
nitric oxide synthase 2, inducible (Inos) | F: TGAATTTGTCAACCTGTATTAC | |||
R: CTTTGTTACCGCTTCCAC | 53 | 82 | NM_001143690.1 | |
Chitinase-3-like protein 1 (Chi3l1) | F: TGACGCTCTATGACACAC | |||
R: GGCTAGGTCCAGTCCATC | 62 | 194 | NM_001001540 | |
Cell Proliferation and Apoptosis | ||||
Caspase-8 (Casp8) | F: TGGGCAAACAGATGCCACAACCT | |||
R: CCCCTTCAATCTAGCCCACCCCC | 60 | 153 | NM_001031779.2 | |
Fas (TNF receptor superfamily, member 6) (Fas) | F: TAGAGTTTGTGATGGAGAA | |||
R: ATTGAGAAGTGTGACAGA | 53 | 107 | NM_213839.1 | |