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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: Sera of patients with axial spondyloarthritis (axSpA) enhance osteoclastogenic potential of monocytes isolated from healthy individuals

Fig. 3

TRAP staining of monocyte-derived osteoclasts. Monocytes were differentiated to osteoclasts for 20 days in presence of serum from axSpA patients and healthy donors as described in “materials & methods”. a-d Cells were fixed and stained to detect TRAP activity. TRAP-positive (violet-labeled) cells with at least three nuclei were considered osteoclasts (blue arrows). e Quantification of osteoclast differentiation efficiency. Graph represent the mean + SD quantified form n = 12 representative micrographs for each condition. (*P = 0.0344; mean values: 3.9 vs. 5.2%). Statistics: One-way ANOVA with Tukey’s correction for multiple testing

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