Fig. 1
High-resolution respirometry with permeabilized fibers from a muscle biopsy sample. Oxygen flux (JO2) is displayed as pmol O2.s− 1.mg− 1 wet weight and changes in response to application of the following substrate-uncoupler-inhibitor titration protocol: mitochondrial leak state without adenylates (LN) after addition of glutamate (G) and malate (M), complex I-supported oxidative phosphorylation capacity (OXPHOS) after addition of ADP (D), pyruvate (P) and cytochrome c (c), complex I-supported electron transfer capacity (ETC) after addition of an uncoupler (U), and succinate-supported ETC after addition of succinate (S), followed by titration of rotenone (Rot); at the end of the protocol, malonic acid (Mna) and antimycin A were added. Abbreviations: CIP = complex I-supported oxidative phosphorylation capacity; CIE = complex I-supported ETC; CIIE = ETC of CII; CI + IIE = maximal ETC of CI and CII; ETC = electron transfer capacity; OXPHOS = oxidative phosphorylation; LN = leak state without adenylates