Table 6 Information of human primers for Real-time PCR
From: The hsa-miR-181a-5p reduces oxidation resistance by controlling SECISBP2 in osteoarthritis
Gene | Sequence (5′-3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
SBP2 | Forward: CCGCAGATTCAGGGATTACT | 92 | 60 |
Reverse: CTTGGAAACGGACCAGTTCT | |||
ACAN | Forward: GGCATTTCAGCGGTTCCTTCTC | 135 | 60 |
Reverse: AGCAGTTGTCTCCTCTTCTACGG | |||
MMP13 | Forward: AATATCTGAACTGGGTCTTCCAAAA | 102 | 60 |
Reverse: CAGACCTGGTTTCCTGAGAACAG | |||
COL2A1 | Forward: TGGACGATCAGGCGAAACC | 244 | 62 |
Reverse: GCTGCGGATGCTCTCAATCT | |||
GPx1 | Forward: AAGCTCATCACCTGGTCTCC | 124 | 60 |
Reverse: CGATGTCAATGGTCTGGAAG | |||
GPx4 | Forward: GCTGTGGAAGTGGATGAAGA | 105 | 60 |
Reverse: TGAGGAACTGTGGAGAGACG | |||
SELS | Forward: CACCTATGGCTGGTACATCG | 130 | 60 |
Reverse: AACATCAGGTTCCACAGCAG | |||
GAPDH | Forward: CACCCACTCCTCCACCTTTG | 110 | 64 |
Reverse: CCACCACCCTGTTGCTGTAG |