Fig. 5
A. The anabolic and catabolic gene expression of NP. mRNA levels of NP in the disc normalized to endogenous control (GAPDH) and internal controls. Relative transcript levels were calculated as χ = 2-ΔΔCt, in which ΔΔCt = ΔE - ΔC, ΔE = Ctexp - CtGAPDH, and ΔC = Ctct1 – CtGAPDH. A~C are shown for anabolic genes in the NP, catabolic genes as shown in the D~F. (⊗) indicates a significant difference from the internal controls (p ≤ 0.05) and (#) indicates that from other groups (one-way ANOVA with Fischer PLSD post hoc p ≤ 0.05), and (*)indicates a significant difference (p ≤ 0.05), and (+) indicates a significant difference (p ≤ 0.001). B. The anabolic and catabolic gene expression of AF. mRNA levels of AF in the disc normalized to endogenous control (GAPDH) and internal controls. Relative transcript levels were calculated as χ = 2-ΔΔCt, in which ΔΔCt = ΔE - ΔC, ΔE = Ctexp - CtGAPDH, and ΔC = Ctct1 – CtGAPDH. A~C are shown for anabolic genes in the AF, catabolic genes as shown in the D~F. (⊗) indicates a significant difference from the internal controls (p ≤ 0.05) and (*)indicates a significant difference (p ≤ 0.05), and (+) indicates a significant difference (p ≤ 0.001)