Fig. 5

The effects of hPTH(1–34) and GR(1–34) on callus transformation and osteoclasts formation in the healing area at 2 weeks after fracture. a The upper two panels showed the Masson-Goldner Trichrome stain for the cartilage (in blue) and bony (in red) callus formation; the third panel showed the immunohistochemical staining with the monoclonal antibody against TRAP; the bottom panel showed the distribution of osteoclasts on the surface of bone stained by TRAP activity. Histomorphometry of callus (in blue and red) and the proportion of bony callus among total callus were statistically shown in (b) and (c), respectively. Statistical analyses were shown for the number of osteoclasts per unit area (d) and their surface in proportion to bone surface (e). [Each group contained 6 cases; scale bar represents 100 μm; *P < 0.05 for hPTH(1–34) &GR(1–34) vs. vehicle; #P < 0.05 for hPTH(1–34) vs. GR(1–34)]