Fig. 4

Analysis of the content and distribution of collagen in transverse sections of bioscaffolds. a Light microscopy images of cellularized or decellularized bioscaffolds in the collected IR spectra (bar = 10 μm). b Spectroscopic maps of the distribution and content of collagen in the cellularized and decellularized bioscaffolds. Red and blue colors indicate high and low matrix content, respectively. c Spectroscopic maps of the distribution and content of proteoglycan in the cellularized and decellularized bioscaffolds. Green and blue colors indicate high and low matrix content, respectively. d Content of collagen within the cellularized or decellularized bioscaffolds. e Content of proteoglycan within the cellularized or decellularized bioscaffolds. Dissimilar letters indicating a significant difference (P < 0.05)