Skip to main content
Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: A comparison of the stem cell characteristics of murine tenocytes and tendon-derived stem cells

Fig. 4

Histological analysis of tri-lineage differentiation potential of tenocytes and TDSCs. Representative images are shown for both cell types after induction of osteogenic, adipogenic and chondrogenic differentiation (positive) and also for control samples (negative), after appropriate staining. Cells subjected to osteogenic differentiation media were stained for both alkaline phosphatase (ALP) activity and calcium deposits using alizarin red (AR). Cells subjected to adipogenic differentiation media were stained for oil droplet formation using oil red O (ORO), and cell pellets exposed to chondrogenic differentiation media, for GAG formation using alcian blue (AB) and safranin O (SO). Bar = 100 μm. Chondrogenic staining was not performed on TDSCs due to low cell numbers. n = 6 biological replicates

Back to article page