Table 2 Four pairs of primers were used to amplify and synthesize the human BMPR-IA gene for the following mutations (MT -349C > T, MT 4A > C) by PCR using pcDNA3.1/BMPR-IA wild type plasmid as the template
SNPs | Primer | Forward | Reverse | Annealing Temperature (°C) |
|---|---|---|---|---|
-349C > T | Primer 1 | CCGGAATTCACCATGGTGGCCGGGACC | CCGACGCCGCCGCCGCGAACTT | 60 |
| Â | Primer 2 | TCGCGGCGGCGGCGTCGG | CCGCTCGAGCTAGCGACACCCACAACCCTCCA | 60 |
4A > C | Primer 1 | CCGGAATTCACCATGGTGGCCGGGACC | CTGGTGTCCAAAAGTCTGGTCACGGG | 60 |
| Â | Primer 2 | CCCCGTGACCAGACTTTTGGACACC | CCGCTCGAGCTAGCGACACCCACAACCCTCCA | 60 |