Genetic polymorphisms in bone morphogenetic protein receptor type IA gene predisposes individuals to ossification of the posterior longitudinal ligament of the cervical spine via the smad signaling pathway

Table 1 Ten pairs of primers were used to amplify the complete exon sequences of human BMPR-IA gene by PCR using a standard protocol

Primer	Forward	Reverse	Annealing temperature (°C)
Primer1	GCGTTGGATGGGAGCGATAA	GGAAGCTGCGCACAGTGTTG	58
Primer2	CTCACGTCGGTCCTGTCC	CCCTGCTCCATGCCTCAC	61
Primer3	CGGAGGAGTTTATCACCTCAGCAGAGC	TTCCATCATGGCCAAAAGTTACTAGCA	60
Primer4	AGAGATTGGAATCCGCCTGCCGGGCTT	ACCCGCGAGTGGGAGACAAAAGAGG	55
Primer5	GGACTATTGAGATTGTTTAATATAC	GAAAGAACAGAAGCAAGAAATAGTG	55
Primer6	GGACTATTGAGATTGTTTAATATAC	GAAAGAACAGAAGCAAGAAATAGTG	55
Primer7	CCACAATGCATCTGGCCCCAAGGAG	TGTGATTATTACACATGGCATGCCTGTATC	55
Primer8	ACATCAGATTACTGGGAGCC	TATAGCAAAGCAGCTGGAG	52
Primer9	AAGCCTTAAGAAGATAAATGA	ATTACCCTAATGAAGTTTTTG	62
Primer10	TGATTAGTGTCTCCAGTCAAGC	TCTCAGGTAAAAGGCAAAGTC	50

ISSN: 1471-2474