Fig. 7From: Genetic polymorphisms in bone morphogenetic protein receptor type IA gene predisposes individuals to ossification of the posterior longitudinal ligament of the cervical spine via the smad signaling pathwayWestern blot analysis of Smad4 protein levels in the transfected C3H10T1/2 cells. Lane 1 C3H10T1/2 cells; Lane 2 C3H10T1/2/pcDNA3.1 cells; Lane 3 C3H10T1/2/pcDNA3.1/BMPR-IA (WT) cells; Lane 4 C3H10T1/2/pcDNA3.1/BMPR-IA (MT -349C > T) cells; Lane 5 C3H10T1/2/pcDNA3.1/BMPR-IA (MT 4A > C) cells; Lane 6 C3H10T1/2/pcDNA3.1/BMPR-IA (MT -349C > T and 4A > C) cells. There was no significant differences in Smad4 protein levels among the experimental groups (n = 5)Back to article page