Skip to main content
Fig. 6 | BMC Musculoskeletal Disorders

Fig. 6

From: Genetic polymorphisms in bone morphogenetic protein receptor type IA gene predisposes individuals to ossification of the posterior longitudinal ligament of the cervical spine via the smad signaling pathway

Fig. 6

The proteins levels of phosphorylated Smad1/5/8 in the transfected C3H10T1/2 cells were detected by Western blotting. a Western blot analysis of phosphorylated Smad1/5/8 proteins levels in transfected C3H10T1/2 cells. Lane 1 C3H10T1/2 cells; Lane 2 C3H10T1/2/pcDNA3.1 cells; Lane 3 C3H10T1/2/pcDNA3.1/BMPRIA (WT) cells; Lane 4 C3H10T1/2/pcDNA3.1/BMPR-IA (MT -349C > T) cells; Lane 5 C3H10T1/2/pcDNA3.1/BMPR-IA (MT 4A > C) cells; Lane 6 C3H10T1/2/pcDNA3.1/BMPR-IA (MT -349C > T and 4A > C) cells. b Statistical analysis of phosphorylated Smad1/5/8 proteins levels in C3H10T1/2 cells, C3H10T1/2/pcDNA3.1 cells, C3H10T1/2/pcDNA3.1/BMPR-IA (WT) cells, and C3H10T1/2/pcDNA3.1/BMPR-IA (MT -349C > T, MT 4A > C, MT -349C > T and 4A > C) cells using the linear density ratio of phosphorylated Smad1/5/8/GAPDH. The phosphorylated Smad1/5/8 proteins levels were significantly increased in C3H10T1/2/pcDNA3.1/BMPR-IA (MT -349C > T, MT -349C > T and 4A > C) cells compared to C3H10T1/2/pcDNA3.1/BMPR-IA (WT) cells, C3H10T1/2/pcDNA3.1 cells, and C3H10T1/2 cells. n = 5, *p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA WT cells compared with C3H10T1/2 cells, *p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA WT cells compared with C3H10T1/2/pcDNA3.1 cells; **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T cells compared with C3H10T1/2 cells, **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T cells compared with C3H10T1/2/pcDNA3.1 cells; *p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT 4A > C cells compared with C3H10T1/2 cells, *p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT 4A > C cells compared with C3H10T1/2/pcDNA3.1 cells; **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T cells compared with C3H10T1/2 cells, **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T and 4A > C cells compared with C3H10T1/2/pcDNA3.1 cells; **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T cells compared with C3H10T1/2/pcDNA3.1/BMPRIA WT cells, **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T and 4A > C cells compared with C3H10T1/2/pcDNA3.1/BMPRIA WT cells. The protein levels of phosphorylated Smad1/5/8 were not increased significantly in C3H10T1/2/pcDNA3.1/BMPR-IA (MT 4A > C) cells compared to the C3H10T1/2/pcDNA3.1/BMPR-IA (WT) cells (n = 5)

Back to article page

BMC Musculoskeletal Disorders

ISSN: 1471-2474

Contact us