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Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: Genetic polymorphisms in bone morphogenetic protein receptor type IA gene predisposes individuals to ossification of the posterior longitudinal ligament of the cervical spine via the smad signaling pathway

Fig. 4

The BMPR-IA gene coding sequence and pGEM-T/BMPR-IA (WT) vector digested with Hind III/Bam HI enzyme were analyzed by 4% agarose gel electrophoresis, respectively. a The annealed ds-oligos of BMPR-IA gene coding sequence was analyzed by 4% agarose gel electrophoresis. Each ds oligo annealing reactant showed a detectable molecular weight band around 1599 bp, as expected for the length of the designed ds-oligos of BMPR-IA gene. Lane M: Marker, Lane B: BMPR-IA gene.0020b After the pGEM-T/BMPR-IA (WT) vector had been digested with Hind III/Bam HI enzyme, the fragments for BMPR-IA cDNA (1599 bp) and the pGEM-T vector (3000 bp) were observed in all the PCR products as analyzed by 4% agarose gel electrophoresis, respectively. The results showed that the ds-oligos of the BMPR-IA gene cDNA were ligated into the pGEM-T vector. Lane M: Marker, Lane 1: pGEM-T/BMPR-IA (WT) vector, Lane 2: The pGEM-T vector and BMPR-IA cDNA

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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