Fig. 7
Expression of Angpt1, Angpt2, eNOS, iNOS, VEGFa, Pecam1, Kdr, CD34, Fgf2 and Fgfr1 and vessel density during muscle regeneration in control and Li-SWT mice. a Angpt2, eNOS and iNOS were high expressed on day 2 in Li-SWT, with eNOS being significantly higher expressed in the Li-SWT group. Angpt1, eNOS, iNOS, VEGFa and Pecam1 were significant up regulated on day 21 in the Li-SWT group. Thus Li-SWT induced an early and late angiogenic signal. No effect of Li-SWT was found in expression of Kdr, CD34, Fgf2, but Fgfr1 was significantly increased in the Li-SWT group on day 2. b and (c) Blood vessels were visualized by immunostainings for vWF and the vessel density (number of vessels/cm2) peaked on day 3 in regenerating areas before decreasing again. No effect of Li-SWT on vessel density was found in either regenerating- or normal muscle areas. c Immunostainings for vWF are shown for day 3 in Li-SWT and controls.n = 3 in gene expressions and n = 4 in quantifications, * p < 0.05