Fig. 1
Quantifications of cell death, total cell counts, BrdU incorporation in cell cultures and gene expressions of TGFB1, SMAD3, SMAD7, KI67, CYCLIN D1, P21, P53 and CASP3 during myoblast proliferation. a Viability testing by tryphan blue at 300500, 1000 and 1500 impulses (0.1 mJ/mm2, 5hz) with 3 human cell cultures showed a small, but insignificant, increase in cell death after Li-SWT. b The myoblast proliferation was assessed in controls and after Li-SWT by detaching and counting the cells every day for 4 days. Variation between cell cultures was found, but no effect of Li-SWT was observed. c Proliferation was further investigated by BrdU incorporation followed by immunostainings, which revealed a 2% increase in proliferation on day 1 after Li-SWT. d Gene expression study during proliferation revealed that TGFB1 expression was significant lower at 12 h after Li-SWT while SMAD7 was higher expressed in Li-SWT compared to controls. P21 was significant different at 12 h, 24 h and 72 h while SMAD3, KI67, CYCKLIND1, P53 and CASP3 showed no difference between treatment groups.n = 3, * p < 0.05