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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: Sequential activation of the AKT pathway in human osteoblasts treated with Oscarvit: a bioactive product with positive effect both on skeletal pain and mineralization in osteoblasts

Fig. 3

Time course of mineralization. a Osteoblasts (2 × 106 each) were grown in MEM supplemented with OSC (20 μg/ml) over a period of 5 days (T1), 10 days (T2), 15 days (T3) and 20 days (T4) in comparison to control cells (C1, C2, C3, C4) grown under identical conditions except for OSC. b Measurement of LDH activity. After a 15-day cultivation period of each of 2 × 106 osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-×100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate

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