Fig. 7

a, b, c, d, e Cytokine analysis assessed by the Luminex assay. Serum samples of mice with an isolated femur fracture (Fx) and mice with chest trauma and femur fracture (TTFx) were analysed by multiplex immunoassay. All Data are shown as box-and-whisker plot with median and interquartile range. Concentration of different cytokines (IL-1β, IL-6, IL-10, IL-12p70 and TNFα) was measured in different groups of mice 0 h, 6 h, 12 h, 3 d and 7 d (n = 8) after trauma. Figures show regulation of IL-1α, IL-6, IL-10, IL-12p70 and TNFα after an isolated femur fracture from 0 h to 7 d after trauma and cytokine regulation after induction of chest trauma and femur fracture from 0 h to 7 d after trauma. Cytokine expression was compared respectively to Sham group