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Fig. 6 | BMC Musculoskeletal Disorders

Fig. 6

From: Bilateral muscle fiber and nerve influences by TNF-alpha in response to unilateral muscle overuse – studies on TNF receptor expressions

Fig. 6

a-c Parallel sections of muscle fibers, processed with ISH for TNFR1 mRNA (a) and immunohistochemistry (IHC) with antibodies towards desmin (b) and NK-1R (c). Please note that (c) is in higher magnification than (a) and (b). a shows that there is an abnormal muscle fiber expressing TNFR1 mRNA in the middle, in which in (b) a high expression of desmin is seen. In (c), there is an existence of point-like NK-1R immunoreactions in this muscle fiber (arrows). The muscle fiber is not filled with fine cells of the type seen in Fig. 5. Also the muscle fiber to the right is to some extent abnormal, partly showing high expression of desmin (b). The muscle fiber below in (b) shows a more normal, striated, desmin pattern. Sample from experimental animal (6 week group, contralateral side). Asterisk at corresponding location. Orig. magnif. ×300. d-f Sections of specimen of 6 week experimental group (contralateral side) processed with antisense and sense TNFR1 mRNA probes (d, e) and for desmin IHC (f) (in f, processing for DAPI was performed). Some of the muscle fibers appear abnormal, several of them being small in size (asterisks), whilst others appear normal (M1, M2). Note the presence of TNFR1 mRNA (white arrows, d) in the abnormally appearing small muscle fibers but not in those with normal morphology. Note also the presence of internal nuclei in the abnormal muscle fibers (white arrowheads, f). As in a-c the abnormal muscle fibers are not filled with fine cells of the type seen in Fig. 5. There are no specific reactions in (e). Orig. magnif. ×300

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