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Fig. 13 | BMC Musculoskeletal Disorders

Fig. 13

From: Bilateral muscle fiber and nerve influences by TNF-alpha in response to unilateral muscle overuse – studies on TNF receptor expressions

Fig. 13

a, b Double staining (IHC) of nerve fascicle with antibodies towards TNFR1 (green colour) (a) and βIII-tubulin (merged reactions in b). There is co-localization between TNFR1 and βIII-tubulin IR in nerve profiles in (b) (arrows). Sample from experimental group (6 week group; contralateral side). Orig. magnif. ×500. c. Double staining for TNFR1/Schwann cell marker S-100β: Small part of a nerve fascicle is shown. There are TNFR1 reactions (green-yellowish) in one of the Schwann cells (red) (arrowhead). Sample from experimental group (6 week group; experimental side). Orig. magnif. ×500. d, e. Double staining TNFR2/S-100β [TNFR2 reaction in green in (d), merged reactions in (e); S-100β in red)]. There is TNFR2 IR in S-100β stained cells, i.e. Schwann cells. Arrows at the TNFR2 immunoreactive spots. Sample from 6 week group, contralateral side. Orig. magnif. ×500

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