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Fig. 2 | BMC Musculoskeletal Disorders

Fig. 2

From: Co-expression of 1α-hydroxylase and vitamin D receptor in human articular chondrocytes

Fig. 2

Cartilage (a, 400×), normal colon (b, 400×), cultured chondrocytes (c) and two-day spheroids (d, 100×) labelled with a VDR antibody. In normal colon the brown staining of the cytoplasm represent VDR labelling and in cultured chondrocytes the VDR is correspondingly visualised by red staining, while in cartilage and two-week spheroids there is no labelling of VDR. Western blot (e) of cartilage, monolayer chondrocytes and osteoblasts labelled with VDR antibody (53 kD) and β-actin (45 kD). PCR (f) of cartilage, monolayer chondrocytes and spheroids (3D) using primers targeting VDR (336 bp) and APRT (300 bp). Images represent the outcome of investigation of cells and tissues from three different donors

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