Fig. 1
From: Co-expression of 1α-hydroxylase and vitamin D receptor in human articular chondrocytes
Cartilage at 100 × (a) and 400 × (b) magnification, arrows indicate chondrocytes labelled with 1α-hydroxylase antibody and corresponding green labelling of cultured chondrocytes (c). In the two-week spheroids (d, 100×) brown staining of chondrocytes indicate the presence of 1α-hydroxylase. Western blot (e) of cartilage, monolayer chondrocytes and osteoblasts labelled with 1α-hydroxylase antibody (58 kD) and β-actin (45 kD, loading control). PCR (f) of cartilage, monolayer chondrocyte and spheroids (3D) using primers targeting 1α-hydroxylase (521 bp) and APRT (300 bp, quality control). Images represent the outcome of investigation of cells and tissues from three different donors