Fig. 5

The mRNA and protein expression of osteogenic or adipogenic differentiation markers of SMMSCs and MMSCs infected by Lentivirus OGN compared with control groups. a, b Western blotting were employed to examine the protein expression of osteogenic markers genes RUNX2, Ocn, ALP, Wnt5b and adipogenesis marker genes AP2, PPARγ2 of SMMSCs infected by Lentivirus OGN compared with control groups at days 1, 3, 6 and 9. c The quantitative expression of adipogenesis markers gene AP2 and PPARγ2 were measured by RT-qPCR at days 1, 3, 6 and 9 after induction of adipogenic differentiation in SMMSCs infected by Lentivirus OGN. Uninfected cells as control group. d The quantitative expression of osteogenic markers gene RUNX2, Ocn, ALP, Colla1 and Wnt5b were measured by RT-qPCR at days 1, 3, 6 and 9 after induction of adipogenic differentiation in SMMSCs infected by Lentivirus OGN. Uninfected cells as control group. After differentiation 1 day served as controls as 1. e The quantitative expression of adipogenesis markers gene at days 1 and 14 after induction of adipogenic differentiation in MMSCs infected by Lentivirus OGN. f The quantitative expression of osteogenic markers gene at days 1 and 14 after induction of adipogenic differentiation in MMSCs infected by Lentivirus OGN. Each assay was performed in three independent experiments. Data are expressed as mean ± SD from all experiments, as indicated * P < 0.05 and ** P < 0.01