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Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: Effects of Osteoglycin (OGN) on treating senile osteoporosis by regulating MSCs

Fig. 4

Influence of Lentivirus OGN on cell osteogenic differentiation in SMMSCs and MMSCs. A Alizarin-Red staining after osteogenic differentiation at 2, 5, 9 and 14 days in osteogenic medium. Compared with control group (A upper line), Lentivirus OGN enhanced the mineralization ability of osteoporotic SMMSCs (A lower line). B The differentiation was induced in SMMSCs (as control group, C [a-d]) and infected by Lentivirus OGN (as experimental group, C [e-h]) for 12 days in osteogenic medium and progress of osteogenic differentiation was explored by ALP staining at day 2 (b, f), 5 (c, g) and 9 (d, h). C Quantification of A was performed by optic density (O.D.) measurement at O.D. 450 nm (n = 3). D Quantification of B was performed by ALP activity. E Quantification of the differentiation was induced in MMSCs infected with Lentivirus OGN by ALP staining. Each assay was performed in three independent experiments. Data are expressed as mean ± SD from all experiments, as indicated * P < 0.05

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