Fig. 4

Effects of PAI-1 deficiency on osteoblast differentiation in mouse osteoblasts and bone marrow cells. a Primary osteoblasts were obtained from 3-day-old female WT and PAI-1 KO mice. Total RNA was extracted from primary osteoblasts cultured with or without 10 ng/mL IL-1β or 1 ng/ml TGF-β₁ for 24 h. The levels of Runx2, Osterix, ALP, Col-1, OCN or GAPDH mRNA were assessed by real-time PCR. Data represent the mean ± SEM. n = 6 for each group. *p < 0.05. b Primary osteoblasts were obtained from 3-day-old female WT mice. Total RNA was extracted from primary osteoblasts cultured with or without 10 ng/mL IL-1β or 1 ng/ml TGF-β₁ in the presence or absence of 1 μM SB421542 for 24 h. The levels of Osterix or GAPDH mRNA were assessed by real-time PCR. Data represent the mean ± SEM. n = 6 for each group. **p < 0.01. c Bone marrow cells were obtained from 8-week-old female WT and PAI-1 KO mice. Total RNA was extracted from the bone marrow cells cultured with or without 200 ng/ml BMP-2 or 10 ng/mL IL-1β for 48 h. The levels of Osterix, ALP, OCN or GAPDH mRNA were assessed by real-time PCR. Data represent the mean ± SEM. n = 5 for each group. **p < 0.01 and *p < 0.05