Gene expression profiling in patients with polymyalgia rheumatica before and after symptom-abolishing glucocorticoid treatment

Table 6 qRT-PCR primer sequences

Gene	Sense	Antisense
ACTA1	GCCGTGTTCCCGTCCATCGT	TTCAGGGTCAGGATACCTCTCTTGCT
BDNF	GAGGGGAGCTGAGCGTGTGTG	TTTTTGTCTGCCGCCGTTACCC
COL5A1	CGCCGACACCTCCAACTCCTC	CTCAGTGAACTCCCCCTCCAA
DES	CCATCCAGACCTACTCTGCCCTC	TTGGTATGGACCTCAGAACCCCTTT
EIF4B	CGTCAGCTGGATGAGCCAAAA	GTCCTCGACCGTTCCCGTTCC
IL6	GAGGCACTGGCAGAAAACAACC	CCTCAAACTCCAAAAGACCAGTGATG
MARK4	AGATCCCAGAGCGGCGGAAG	GGGTCATCATGCTAGGAGGGAGGTT
MTFP1	AAGGCAAGAAGGCTGGAGAGGTG	ACAGAGGCTAGAGCCTGCCATACAAA
NPM1	GGTTTCCCTTGGGGGCTTTG	GCACTGGCCCTGAACCACACTT
PRSS23	CAGCGGGTCTGGGGTCTATG	GCCAATAATTTTTCGCTCCCACTTCT
TUBD1	TGATTGTTGGGAAGGCATGGA	CAACAACCTGCTCTAATGACGTGAAA
TFRC	TCGGGAATGCTGAGAAAACAGACA	TTTTGGAGATACGTAGGGAGAGAGGAA
TNFA	TTCCCCAGGGACCTCTCTCTAATC	GAGGGTTTGCTACAACATGGGCTAC
TUBA8	GCCCAAGGATGTGAATGTCGCT	GGTCGGGGGCTGGTAGTTGATG
RPLP0	GGAAACTCTGCATTCTCGCTTCCT	CCAGGACTCGTTTGTACCCGTTG

qRT-PCR quantitative real-time PCR
The primer set sequence for BDNF provided in Table 6 recognizes all BDNF isoforms; using this primer set, the results presented in Table 5 were obtained. The BDNF mRNA levels were also assessed with qRT-PCR using a BDNF primer set that specifically recognizes the BDNF isoform that is recognized by the probe on the used microarray; the results (fold difference + 1.53, p < 0.1; fold change −2.4, p < 0.01) from this additional assessment were very similar to the results presented in Table 5

ISSN: 1471-2474