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Table 5 Quantitative RT-PCR fold differences between untreated patients with polymyalgia rheumatica (PMR) and non-PMR controls, and fold changes between treated and untreated PMR patients

From: Gene expression profiling in patients with polymyalgia rheumatica before and after symptom-abolishing glucocorticoid treatment

Gene symbol Fold differencesb Fold changesc
(probe name) qRT-PCR Microarraya qRT-PCR Microarraya
Genes that differed in microarray testing in at least one comparison
BDNF +1.90* +1.80 * −1.58 ** −3.1 **
COL5A1 −1.33 ns −1.30 ** +1.73 ns +2.30 ns
EIF4B +1.63 p = 0.0504 +1.30 * −1.23 * −1.30 *
MARK4 +1.32 ns +1.30 ** −1.24 * −1.15 ns
MTFP1 +1.00 ns −1.50 ** +1.33 * +1.30 *
NPM1 +1.38 ** +1.30 * −1.09 ns −1.22 *
PRSS23 −1.21 ns −1.40 * +1.27 ns +1.51 *
TFRC −1.63 ns −3.00 * +1.17 ns +1.76 ns
TUBD1 +1.26 ** +1.30 ** −1.08 ns −1.40 **
Genes that did not differ in microarray testing
ACTA1 (203872_at) −1.03 ns −1.02 ns 1.06 ns +1.00 ns
DESa (216947_at 202222_s_at 214027_x_at) +1.16 ns +1.00 ns −1.07 ns +1.00 ns
IL6 (205207_at) +4.54 * +1.02 ns −3.25 * +1.02 ns
TNFA (207113_s_at) +1.31 ns +1.00 ns −1.31 ns −1.00 ns
TUBA8 (220069_at) −1.02 ns −1.02 ns +1.10 ns +1.00 ns
  1. qRT-PCR quantitative real-time PCR
  2. * p < 0.05. ** p < 0.01. ns, not statistically significant. Data are geometric means
  3. a Microarray numbers were calculated as the mean of the individual probe values
  4. b + and −, expression levels were higher and lower, respectively, in patients with polymyalgia rheumatica than in controls before treatment with prednisolone
  5. c + and −, expression levels increased and decreased, respectively, in patients with polymyalgia rheumatica after treatment with prednisolone