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Table 1 Clinical details of the patient

From: Sonication contribution to identifying prosthetic joint infection with Ralstonia pickettii: a case report and review of the literature

Date and type of surgery

November 2014 Debridement, and retention of fixed prosthetic components

March 2016 Debridement, explanation of the prosthesis, and implantation of preformed spacer loaded with gentamicina

June 2016 Internal Medicine Department Hospitalization

July 2016 Debridement, and changing of the preformed spacer loaded with gentamicin

September 2016 Debridement, and changing of the preformed spacer loaded with gentamicin

February 2017 Debridement, and reimplantation of a revision prosthesis

Blood sugar mg/dl (R.V. 83–110)

190

139

139

109

239

142

Fibrinogen mg/dl (R.V. 180–400)

530.5

424.7

438.2

523.2

560

388.9

WBCs (R.V. 4–10 × 103/μl)

8.46

8.9

8.33

11.07

9.11

4.96

HGB (R.V.13-17 g/dl)

10.5

11.6

11.5

11.0

9.4

11.7

NEUT (R.V. 2–7.5 × 103/μl)

6.16

6.1

6.47

8.38

7.61

3.31

ESR (R.V. 0-15 mm/h)

69

27

22

47

46

16

C-reactive protein (R.V. <6 mg/dl)

NA

27

22

24

24

6

Joint fluid hs-CRP (R.V. 0.00–0.50 mg/dl)

NA

NA

-

NA

0.78

0.89

Histopathology Resultsb

Type II

Type III

-

Type II

Type II

Type II

Culture results

Preoperative samples (joint fluid)

Negative

Negative

-

Negative

Negative

Negative

Intraoperative samples (tissue biopsies – 3 samples)

Negative

Pseudomonas aeruginosa (in all samples)

-

Negative

Pseudomonas aeruginosa (in 2 samples)

Pseudomonas aeruginosa (in 1 sample)

Sonication fluid culture

NA

NA

-

NA

Negative

Ralstonia pickettii

Molecular identification of bacteria by 16S rRNA bbFISH® technologyc

On Sonication Fluid

NA

NA

-

NA

Negative

Negative

  1. aSigns of femoral and acetabular component loosening were present; b according to the Krenn and Morawietz [2]: type I periprosthetic membrane (ppm) of the wear particle-induced type, type II ppm of the infectious type, type III ppm of the combined type, type IV indeterminate type; c Beacon-based fluorescent in situ hybridization kit (hemoFISH® Masterpanel, miacom diagnostics GmbH Düsseldorf, Germany); R.V references values, WBC White blood cells, HGB hemoglobin, NEUT neutrophils, ESR erythrocyte sedimentation rate, NA not available