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Table 2 Effect of TNF-α and TAK1 inhibitor on MMP-3, ADAMTS-4, COX-2, mPGES1, and NGF mRNA expression

From: Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain-related molecule expression following tumor necrosis factor-α stimulation of synovial cells: an in vitro study

Gene

Fold increase

p

Control

TNF-α

TNF-α + 5Z

Control vs TNF-α

TNF-α vs TNF-α +5Z

Control vs TNF-α +5Z

ADAMTS-4

1.0 ± 0.1

2.0 ± 0.1a

0.5 ± 0.1a,b

0.0003

3 × 10−4

0.031

MMP-3

1.0 ± 0.3

1.6 ± 0.2a

0.6 ± 0.1b

0.018

0.028

0.096

COX-2

1.0 ± 0.3

11.2 ± 2.6a

4.5 ± 1.5b

0.007

0.014

0.065

mPGES1

1.0 ± 0.3

3.1 ± 0.5a

1.5 ± 0.5b

0.024

0.003

0.483

NGF

1.0 ± 0.1

2.7 ± 0.2a

0.8 ± 0.2b

0.006

0.003

0.312

  1. Real-time polymerase chain reaction analysis for matrix metalloproteinase-3 (MMP-3), a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4 (ADAMTS-4), cycloxygenase-2 (COX-2), microsomal prostaglandin E synthase-1, and nerve growth factor (NGF) gene expression in synovial cell culture. Synovial cells were stimulated with human recombinant 10 ng/mL TNF-α (TNF-α), or 10 ng/ml TNF-α and 10 μM (5Z)-7-oxozeaenol (TNF-α + 5Z) for 24 h prior to the extraction and analysis of total RNA. All data are presented as the mean ± standard error (n = 6). a p < 0.05 compared with the untreated control. b p < 0.05 compared with the TNF-α