Gene | Fold increase |
p
|
---|
Control | TNF-α | TNF-α + 5Z | Control vs TNF-α | TNF-α vs TNF-α +5Z | Control vs TNF-α +5Z |
---|
ADAMTS-4 | 1.0 ± 0.1 | 2.0 ± 0.1a
| 0.5 ± 0.1a,b
| 0.0003 | 3 × 10−4
| 0.031 |
MMP-3 | 1.0 ± 0.3 | 1.6 ± 0.2a
| 0.6 ± 0.1b
| 0.018 | 0.028 | 0.096 |
COX-2 | 1.0 ± 0.3 | 11.2 ± 2.6a
| 4.5 ± 1.5b
| 0.007 | 0.014 | 0.065 |
mPGES1 | 1.0 ± 0.3 | 3.1 ± 0.5a
| 1.5 ± 0.5b
| 0.024 | 0.003 | 0.483 |
NGF | 1.0 ± 0.1 | 2.7 ± 0.2a
| 0.8 ± 0.2b
| 0.006 | 0.003 | 0.312 |
- Real-time polymerase chain reaction analysis for matrix metalloproteinase-3 (MMP-3), a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4 (ADAMTS-4), cycloxygenase-2 (COX-2), microsomal prostaglandin E synthase-1, and nerve growth factor (NGF) gene expression in synovial cell culture. Synovial cells were stimulated with human recombinant 10 ng/mL TNF-α (TNF-α), or 10 ng/ml TNF-α and 10 μM (5Z)-7-oxozeaenol (TNF-α + 5Z) for 24 h prior to the extraction and analysis of total RNA. All data are presented as the mean ± standard error (n = 6). a
p < 0.05 compared with the untreated control. b
p < 0.05 compared with the TNF-α