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Fig. 6 | BMC Musculoskeletal Disorders

Fig. 6

From: Impairment of early fracture healing by skeletal muscle trauma is restored by FK506

Fig. 6

Evaluation of immune cells infiltrating fracture callus of OST + VML treated with FK506 using immunohistological staining. Injured tibiae were collected at 3 and 14 days post-injury from OST + VML injured groups that received saline or FK506 treatment. Tissue was sectioned and immunohistochemical analysis was carried out to detect CD68+ macrophages, CD4+ T cells, and CD8+ cytotoxic T cells, using horseradish peroxidase for detection. a Representative images from the fracture callus site for each treatment group at the 14 day time point is presented, with magnified inserts presented adjacent to image and identified by a box in the primary image. Scale bars = 1 mm and 50 μm, respectively. b-d The relative area of the fracture callus populated by CD68+, CD4+, and CD8+ cells were quantified. Group responses are presented as floating box plots (minimum to maximum with median line); n = 3/group. * saline >3 day saline & 14 day FK506; p < 0.05. TPI, time post-injury

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