Fig. 5

Flow cytometric analysis of macrophages infiltrating skeletal muscle after OST + VML treated with FK506. TA muscles were isolated from OST + VML groups treated with saline or FK506 at 3 days post-injury to evaluate immune cell infiltration by flow cytometry. The middle third region of the TA muscle was collected, digested, and cellular content isolated. a Events were gated (red polygons) to eliminate debris and doublets, and cells were then gated for CD45⁺ hematopoietic cells, CD45⁺CD11b⁺CD68⁺ macrophages, CD45⁺CD11b⁺CD68⁺CD86⁺ M1-like macrophages (black bar), and CD45⁺CD11b⁺CD68⁺CD163⁺ M2-like macrophages (black bar); b-e Data for each cell population are normalized per gram of muscle tissue and presented as box (25 to 75 percentile with median line) and whisker (minimum and maximum response) plots; n = 4/group. *p < 0.05