Fig. 4

Flow cytometric analysis of T lymphocytes infiltrating skeletal muscle after OST + VML treated with FK506. TA muscles were isolated from OST + VML groups treated with saline or FK506 at 3 days post-injury to evaluate immune cell infiltration by flow cytometry. The middle third region of the TA muscle was collected, digested, and cellular content was isolated. a Events were gated (red polygons) to eliminate debris and doublets, and cells were then gated for CD45⁺CD3⁺ T lymphocytes, CD45⁺CD3⁺CD8⁻CD4⁺ T helper lymphocytes, and CD45⁺CD3⁺CD4⁻CD8⁺ cytotoxic T lymphocytes. b-d Data for each cell population are normalized per gram of muscle tissue and presented as box (25 to 75 percentile with median line) and whisker (minimum and maximum response) plots; n = 4/group. *p < 0.05