Fig. 2
From: Leptin differentially regulates chondrogenesis in mouse vertebral and tibial growth plates
Primary cell cultures of TGP and VGP chondrocytes maintained the chondrogenic characteristics. a Bright field images of the dissected TGP and VGP from neonatal wild type mice (left and middle panels). The right panels show the microscopic images of the primary chondrocytes. Scale bar, 30 μm. b Immunofluorescent microscopic images showing TGP and VGP primary chondrocytes stained for collagen IIA, Sox 9, and leptin receptor. The right most panels represent the control images obtained by using only the secondary antibodies for the immunofluorescent imaging of Collagen II, Sox9, and leptin receptor, respectively