Table 2 The Primers for Real-Time Polymerase Chain Reaction
Target gene | Primers sequence |
|---|---|
RUNX-2 | sense 5’-ACGACAACCGCACCATGGT-3’ |
antisense 5’-CTGTAATCTGACTCTGTCCT-3’ | |
alkaline phosphatase (ALP) | sense 5’-TGGAGCTTCAGA AGCTCAACACCA-3’ |
antisense 5’-ATCTCGTTGTCTGAGTACCAGTCC-3’ | |
peroxisome proliferators-activated receptor 2 (PPAR-2) | sense 5’-CGAGGGCGATCTTGACAGGAA -3’ |
antisense 5’-CAGGGGGGTGATGTGTTTGAAC- 3’ | |
adipogenic protein (APP) | sense 5’-CTGTCCAAGTCCAACAGCAA-3’ |
antisense 5’-ACGTTGGCAGCTTTACGTCT-3’ | |
lipoprotein lipase (LPL) | sense 5’-TCCGCGTGATTGCAGAGAGAG-3’ |
antisense 5’-TGCTGCTTCTTTTGGCTCTGACT-3’ | |
aggrecan (Agg) | sense 5’-TGAGGAGGGCTGGAACAAGTACC-3’ |
antisense 5’-GGAGGTGGTAATTGCAGGGAACA-3’ | |
collagen II (Col II) | sense 5’-TTTCCCAGGTCAAGATGGTC-3’ |
antisense 5’-TCACCTGGTTTTCCACCTTC-3’ | |
SOX-9 | sense 5’-TGGCCGAGATGATCCTAAAAATAA -3’ |
antisense 5’-GCGCTTGGATAGGTCATGTTTGT-3’ | |
SOX-2 | sense 5’-CCCCTGTGGTTACCTCTTCCTC-3’ |
antisense 5’-GGCCGCTCTGGTAGTGCTG-3’ | |
NANOG | sense 5’-ACCCCGTTTCACTGTGTTAGC-3’ |
antisense 5’-GACGGCAGCCAAGGTTATTAAA-3’ | |
OCT4 | sense 5’-GGCAAGCGATCAAGCAGCGAC-3’ |
antisense 5’-GGGAAAGGGACCGAGGAGTAC-3’ | |
β-actin | sense 5’-GTGGGGCGCCCCAGGCACCA-3’ |
antisense 5’-CTTCCTTAATGTCACGCACGATTTC-3’ |