Fig. 5

Inflammation-induced gene expression of matrix degrading enzymes and collagen type X was decreased by IL-10. Expression levels of matrix-degrading enzymes were evaluated 72 h after treatment with proinflammatory TNF-α (10 and 100 ng/ml) and anti-inflammatory IL-10 [10 ng/ml]. For each condition eight disks from the same animal were pooled for mRNA extraction (n = 5). Expression levels of a MMP3, b MMP13, c ADAMTS4 and d COL10A1. Gene expression levels were normalized to that of GAPDH house-keeping gene and then normalized to the non-stimulated control group without compression, which had an expression level = 1. a,b indicate clusters of experimental groups which are significantly different from each other with P < 0.05 (detailed p-values are given in the text). Data are presented as mean ± 95 CI