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Fig. 6 | BMC Musculoskeletal Disorders

Fig. 6

From: Pigment Epithelium-Derived Factor (PEDF) mediates cartilage matrix loss in an age-dependent manner under inflammatory conditions

Fig. 6

PEDF-deficiency protects against monosodium iodoacetate (MIA)-induced joint cartilage damage. a Immunofluorescence staining was used to determine PEDF protein levels in knee joints from 29 week old wild type animals using a mouse anti-PEDF antibody. Nuclei were stained with DAPI. A negative control using goat anti-mouse IgG secondary antibody alone is shown in the Additional file 2: Figure S1. M: meniscus; F: femur; T: tibia. Scale bar = 200 μm. b MIA or PBS was intraarticularly injected into 29 week old wild type or PEDF-deficient knees, which were harvested after 10 days. Samples were stained with Safranin O and counterstained with Hematoxylin and Fast Green. Arrows indicate areas exhibiting a loss of Safranin O staining. Scale bar = 100 μm. c The percent area loss of Safranin O staining along the articular surface was calculated for the femur and tibia independently, as well as for the whole joint (femur + tibia). Each point represents the averaged score of an individual animal. Data are plotted as mean ± SEM. d OARSI scores were calculated from Safranin O stained joints. Each point represents an individual animal. The bar represents the median. e Quantification of percent cell loss based on DAPI cell counts of the articular surface and empty lacunae. ** p < 0.01 (Mann–Whitney test) * p < 0.05 (Student’s t-test)

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