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Fig. 8 | BMC Musculoskeletal Disorders

Fig. 8

From: Anti-fibrotic action of pirfenidone in Dupuytren’s disease-derived fibroblasts

Fig. 8

Pirfenidone inhibits TGF-β1-induced phosphorylation of Smad2/Smad3. CT-and DD-cord-derived fibroblasts derived from three different patient samples (N = 3/group) were maintained in MEM- α medium containing 0.1% dialyzed FBS for 24 h. After 24 h, cells were either treated or untreated with PFD (800 μg/ml) in the presence or absence of TGF-β1 (10 ng/ml) for additional 24 h. Cell lysates collected from CT- (a & b) and DD- derived fibroblasts were used to examine the expression of phosphorylated Smad2/Smad3 by Western blot analysis. Proteins obtained from different patients were processed in parallel to confirm the changes that are observed are consistent. Densitometry results are reported as the ratio of phosphorylated Smad2/Smad3 protein to GAPDH expression (c). Shown here is the representation of Western blot experiments performed using three different culture each of CT- and DD-derived fibroblasts essentially with identical results. *p < 0.05

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