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Fig. 7 | BMC Musculoskeletal Disorders

Fig. 7

From: Anti-fibrotic action of pirfenidone in Dupuytren’s disease-derived fibroblasts

Fig. 7

Pirfenidone substantially reduced TGF-β1 induction of fibronectin expression. CT- and DD-cord derived fibroblasts grown in growth medium overnight were treated with medium containing 0.1% dialyzed FBS for 24 h. Following which, cells were treated with 800 μg/mL of PFD with or without 10 ng/ml TGF-β1 for 24 h. After the treatment, secreted proteins in the growth medium were collected to determine the fibronectin expression using Human Fibronectin SimpleStep ELISA Kit. CT- and DD-derived fibroblasts derived from three different patient samples (N = 3/group) were used to perform the experiment three times in triplicate. Statistical significance was determined by One-way ANOVA. *p < 0.05

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