Fig. 6
From: Anti-fibrotic action of pirfenidone in Dupuytren’s disease-derived fibroblasts
Pirfenidone effectively inhibited TGF-β1-induction of α-2 chain of Type I and α-1 chain of Type III collagen. CT- and DD-derived fibroblasts derived from three independent patient samples (N = 3/group) were left untreated (Ntx) or exposed to PFD (800 μg/ml) in the presence or absence of TGF-β1 for 24 h. Twenty-four hours later RNA was extracted and mRNA expression levels of type I and type III collagen of CT- (a & b) and DD-derived fibroblasts (c & d) were determined using real-time RT-PCR analysis. Shown is the mean ± SEM of n = 3 of experimental replicates from three different culture of CT- and DD-fibroblasts, each performed in triplicate. *p < 0.05, **p < 0.01