Fig. 4

Cellular contractile ability was diminished in the presence of Pirfenidone. CT- and DD-derived fibroblasts derived from four different patient samples (N = 4/group) were left untreated (Ntx) or exposed to PFD (800 μg/ml) in the presence or absence of TGF-β₁ (10 ng/ml) to perform the stressed fibroblast populated collagen lattice (sFPCL) assay. Collagen lattices cultured for 24 h were detached from the surface of the well, and the digital images of the floating lattices were captured at different time points (day 0- day 6). a Shown here are representative images of four independent experiments performed in duplicate. b & c Data were obtained using Adobe Photoshop to analyze photographic images; data are shown as the area of the contracted collagen lattice normalized to the average area of contraction seen in untreated cells, set as a baseline value of 1. Each data point represents the mean ± SEM of the averages of triplicate reads for each culture derived from the four different patient samples both for CT- and DD-derived fibroblasts. Statistical significance was determined using one-way ANOVA. *p < 0.05; **p < 0.01