Fig. 5

Inflammatory response in each group. a H&E staining and immunohistochemistry for F4/80 in the joint. Boxed areas in the top panels are magnified and presented in the bottom panels. Top and middle panels; H&E staining, bottom panel; immunohistochemistry for F4/80 in the synovial membrane. Scale bar: top 250 μm, middle and bottom 25 μm. Number of mice in each group is indicated in the figure. b Histochemical analysis of epiphyseal bone marrow cavity of the femur. Scale bar: 25 μm. Top panels, H&E staining. Bottom panels, F4/80 staining. Number of mice in each group is indicated in the figure. c Semi-quantitative analysis of synovial inflammation. Values are means with SEM (n = 6). Differences among each group were assessed using a Kruskal-Wallis one-way analysis of variance by ranks followed by Steel-Dwass’s post-hoc test. P value is indicated if the difference is statistically significant