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Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms

Fig. 4

Cytokine production and release in response to bacterial biofilm EPS or EPS-derived materials: a Osteoblasts were cultivated for 6 h with either LTA1 (1 μg/mL), GroEL1 or GroEL5 (1 and 5 μg/mL, respectively), or EPS2 (2 % (v/v)) extracted from S. epidermidis biofilms; then cytokine production was determined by quantitative RT-PCR (data of 5 individuals are summarized). b and c show a dose–response curve for GroEL with cells from one individual (mean of duplicates); RT-PCR was performed after 6 h in culture, release into the supernatant after 24 h. df show the summary of experiments with cells of 5–7 individuals. Cytokines were measured in the cell-free supernatant after 24 h stimulation with LTA1 and LTA5 (1 and 5 μg/mL, respectively) (for CCL2, only LTA1 was measured), GroEL1 and GroEL5 (1 and 5 μg/mL, respectively) or EPS2 (2 % (v/v))

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