Fig. 4
a Modulation of miRNA levels in GRMD dog muscles after systemic administration of MuStem cells. Expression levels of miR-1, miR-133a, miR-206, miR-222, and miR-486 were determined in muscles (right and left Biceps femoris) of six 9-month-old GRMDMuStem dogs compared to six mock GRMD dogs. RNU6B was used as internal control and relative expressions are presented as mean ± SEM. *p < 0.05. b miRNA localization in GRMD dog muscle after MuStem cell transplantation. Upper panel: miR-206 expression. Lower panel: miR-486 expression. miR-206 and miR-486 are detected in myoblasts (black arrow head) and in the cytoplasm of small and intermediate regenerating MyHCd+ fibres (asterisk). Scale bar = 25 μm