Skip to main content
Fig. 6 | BMC Musculoskeletal Disorders

Fig. 6

From: The influence of calcitonin gene-related peptide on markers of bone metabolism in MG-63 osteoblast-like cells co-cultured with THP-1 macrophage-like cells under virtually osteolytic conditions

Fig. 6

RANKL protein expression in particle- and LPS-stimulated MG-63 osteoblasts remains unchanged even upon CGRP treatment. MG-63 osteoblasts were stimulated with LPS (100 ng/ml) or UHMWPE particles (cell-to-particle ratio of 1:500) and treated with CGRP (10−8 M). Changes in the expression of both full-length (fl) and soluble (s)RANKL protein were assessed by SDS-PAGE and Western Blot. RANKL protein expression as compared to commercially available LNCaP lysate and recombinant sRANKL as external controls and GAPDH as an internal control is exemplarily shown for cells at 6 and 24 h of incubation. The sRANKL band provided a very strong signal even upon low exposition times so that artifacts can be seen in the neighboring lanes

Back to article page