Fig. 2

The expression of Beclin-1 and LC3 in SW1353 cells. a The protein expression of Beclin-1 and LC3 evaluated by western blotting. b, c Quantitative analysis showed that the protein expression of Beclin-1 and the LC3-II/LC3-I ratio were decreased in IL-1β-induced SW1353 cells compared with that in normal cells. Both the expression levels of Beclin-1 and the LC3-II/LC3-I ratio were much higher in cells treated with IL-1β in combination with Torin 1 than in cells only treated with IL-1β, while they were lower in cells treated with IL-1β in combination with 3-MA. n = 3 for each group. d The expression of LC3 evaluated by immunofluorescence. LC3 was expressed in normal cells and decreased in IL-1β treated SW1353 cells. Moreover, the expression of LC3 was higher in cells treated with IL-1β in combination with Torin 1 and lower in cells treated with IL-1β in combination with 3-MA. n = 3 for each group, and the test was repeated 3 times. *P < 0.05, **P < 0.01, ***P < 0.001