Comparison of cultures and 16S rRNA sequencing for identification of bacteria in two-stage revision arthroplasties: preliminary report

Przemysław Bereza; Alicja Ekiel; Aleksandra Auguściak-Duma; Małgorzata Aptekorz; Iwona Wilk; Damian Kusz; Piotr Wojciechowski; Aleksander L. Sieroń; Gayane Martirosian

doi:10.1186/s12891-016-0991-1

Table 1 Clinical details of patients

From: Comparison of cultures and 16S rRNA sequencing for identification of bacteria in two-stage revision arthroplasties: preliminary report

Patient	Affected joint	CRP before 2^nd stage	Time between 1^st and 2^nd stage (days)	Treatment	Culture result - 1^st stage	Culture results - 2^nd stage			Molecular identification Bacteria identified by 16S rRNA gene sequencing	Followup (mean, 32 months; range, 25–36 months)
Patient	Affected joint	CRP before 2^nd stage	Time between 1^st and 2^nd stage (days)	Treatment	Intraop. specimen	Preop. samples (joint fluid)	Intraop. specimen	Sonicate		Followup (mean, 32 months; range, 25–36 months)
1	H	<5	263	Restoration Stryker	negative	negative	negative	negative	Geobacillus stearothermofilus, G. vulcani	healed
2	H	<5	150	Centrament Aesculap	negative	negative	negative	negative	Lactobacillus jensenii, L. acidophilus, L. fornicalis	death
3	K	<5	146	Scorpio TS Stryker	negative	negative	negative	negative	Pseudomonas aeruginosa, P. resinovorans	healed
4	K	<5	90	Scorpio TS Stryker	negative	negative	negative	Ralstonia pickettii	Novosphingobium nitrogenifigens, N. hassiacum, Bradyrhizobium japonicum, B. liaoningense,	healed
5	H	6,4	145	Restoration Stryker	negative	negative	negative	negative	Klebsiella pneumoniae	healed
6	K	<5	170	Scorpio TS Stryker	negative	negative	negative	negative	Klebsiella pneumoniae	healed
7	K	6	184	Scorpio TS Stryker	Micrococcus sp.	negative	negative	negative	S. lugdunensis, S. hominis	healed
8	K	<5	88	Scorpio TS Stryker	Streptococcus viridans	negative	S. epidermidis	negative	Corynebacterium ureicelerivorans, C. mucifaciens	healed
9	K	<5	150	Scorpio TS Stryker	E.coli	negative	negative	Ralstonia pickettii	Rubrobacter xylanophilus, Clostridium saccharoperbutylacetonicum	healed
10	K	<5	150	Scorpio TS Stryker	Enterococcus faecalis	negative	negative	negative	Tuberibacillus calidus, Bacillus algicola	healed
11	K	27,1	140	Scorpio TS Stryker	Acinetobacter baumani Enterobacter cloacae	negative	negative	negative	negative	failure: prolonged antibiotic therapy (recurrent joint effusion)
12	H	<5	135	Restoration Stryker	Enterococcus faecium	negative	negative	negative	Brevibacterium ravenspurgense, B. paucivorans	healed
13	K	<5	180	Arthrodesis ChM plate	Staphylococcus aureus	negative	S. epidermidis	S. epidermidis	Acinetobacter johnsonii, A. parvus	failure: prolonged antibiotic therapy (prolonged wound healing)

H hip, K knee

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