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Table 1 Clinical details of patients

From: Comparison of cultures and 16S rRNA sequencing for identification of bacteria in two-stage revision arthroplasties: preliminary report

Patient Affected joint CRP before 2nd stage Time between 1st and 2nd stage (days) Treatment Culture result - 1st stage Culture results - 2nd stage Molecular identification Bacteria identified by 16S rRNA gene sequencing Followup (mean, 32 months; range, 25–36 months)
Intraop. specimen Preop. samples (joint fluid) Intraop. specimen Sonicate
1 H <5 263 Restoration Stryker negative negative negative negative Geobacillus stearothermofilus, G. vulcani healed
2 H <5 150 Centrament Aesculap negative negative negative negative Lactobacillus jensenii, L. acidophilus, L. fornicalis death
3 K <5 146 Scorpio TS Stryker negative negative negative negative Pseudomonas aeruginosa, P. resinovorans healed
4 K <5 90 Scorpio TS Stryker negative negative negative Ralstonia pickettii Novosphingobium nitrogenifigens, N. hassiacum, Bradyrhizobium japonicum, B. liaoningense, healed
5 H 6,4 145 Restoration Stryker negative negative negative negative Klebsiella pneumoniae healed
6 K <5 170 Scorpio TS Stryker negative negative negative negative Klebsiella pneumoniae healed
7 K 6 184 Scorpio TS Stryker Micrococcus sp. negative negative negative S. lugdunensis, S. hominis healed
8 K <5 88 Scorpio TS Stryker Streptococcus viridans negative S. epidermidis negative Corynebacterium ureicelerivorans, C. mucifaciens healed
9 K <5 150 Scorpio TS Stryker E.coli negative negative Ralstonia pickettii Rubrobacter xylanophilus, Clostridium saccharoperbutylacetonicum healed
10 K <5 150 Scorpio TS Stryker Enterococcus faecalis negative negative negative Tuberibacillus calidus, Bacillus algicola healed
11 K 27,1 140 Scorpio TS Stryker Acinetobacter baumani Enterobacter cloacae negative negative negative negative failure: prolonged antibiotic therapy (recurrent joint effusion)
12 H <5 135 Restoration Stryker Enterococcus faecium negative negative negative Brevibacterium ravenspurgense, B. paucivorans healed
13 K <5 180 Arthrodesis ChM plate Staphylococcus aureus negative S. epidermidis S. epidermidis Acinetobacter johnsonii, A. parvus failure: prolonged antibiotic therapy (prolonged wound healing)
  1. H hip, K knee
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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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