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Fig. 8 | BMC Musculoskeletal Disorders

Fig. 8

From: Surgical removal and controlled trypsinization of the outer annulus fibrosus improves the bioactivity of the nucleus pulposus in a disc bioreactor culture

Fig. 8

Immunohistochemistry and western blot analysis of protein expression. Photomicrographs a-l show immunohistochemical staining for collagen II and aggrecan within the nucleus pulposus (NP) in the EG and CG at days 7 and 14. b, h: fresh NP. a, g: negative controls. Magnification: 200x, scale represents 100 μm (n = 3). The results (m-o) of the western blot analysis of aggrecan degradation products (ARGxx) and collagen II within the NP in the EG and CG at days 7 and 14. Fresh samples were also used for comparison. CG: control group; EG: experimental group. Data are expressed as the mean ± SD, n = 3. *: p < 0.05 vs. the CG

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