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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: Surgical removal and controlled trypsinization of the outer annulus fibrosus improves the bioactivity of the nucleus pulposus in a disc bioreactor culture

Fig. 3

Histological observation of pretreated discs and cultured discs. The upper half depicts the gross disc appearance and the HE staining of the annulus fibrosus (AF) and the cartilage endplate (CEP) in the EG and CG after pre-treatment. B, B1: the superficial AF; C, C1: the inner AF. D, D1: cross section; E, E1: sagittal section. The lower photomicrographs show the analyses of HE staining (b-e) and toluidine blue staining (b1-e1) in the nucleus pulposus (NP) tissue of the EG and CG at days 7 and 14. Fresh samples (a, a1) were also used for comparison. CG: control group; EG: experimental group. Magnification: B-E, B1-E1 and a-e: 200x, scale represents 100 μm (n = 3); a1-e1: 100x, scale represents 200 μm (n = 3)

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